Technical Data
Media
Deoxycholate Citrate Lactose Sucrose (DCLS) Agar (Powder)
D3172-10
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Peptic Digest of Animal Tissue3.5
Lactose5.0
Casein Digest Peptone3.5
Sucrose5.0
Sodium Thiosulfate5.0
Sodium Citrate10.5
Neutral Red0.03
Sodium Deoxycholate2.5
Agar12.0
Total:47g/liter
Presence of lactose and sucrose in medium reduces incidence of false positive results. Used to recover Salmonella and Shigella.

Description:
DCLS Agar medium is a modification of a formula described by Leifson for the isolation of Enteric pathogens. The addition of both lactose and sucrose in the formula reduces the incidence of false positive results since slow lactose fermenters ferment sucrose readily. The production of acid from the sugar fermentations lowers the pH of the medium and is detected by neutral red. The colony fermenting the sugar takes on a red or deep red color. It is a selective medium because it contains sodium citrate and sodium deoxycholate which inhibit the growth of Gram positive bacteria.

Storage:
Store the sealed bottle containing the dehydrated medium at 2 to 30.0C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light pinkish-beige color.


Directions: 1. Dissolve 47grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

Do not autoclave Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Microbiological Analysis: Escherichia coli ATCC 25922

Appearance: Light pinkish-beige, homogeneous, free flowing powder.

Solubility: Orange-red, clear, complete.

pH (25C): 7.20.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.