Technical Data
D3191
2’-Deoxynucleotide Triphosphates (Set of 4 dNTP’s) dATP, dCTP, dGTP, dTTP Solution, BioGenomics™
4x50uMole
Molecular Biology Storage: -20°C/-70°CShipping: Blue Ice
Suitable for use in PCR™, cDNA synthesis, DNA sequencing and labeling procedures.
Supplied as a set of 4 dNTP’s at a concentration of 100mM.
Functionally tested in PCR with Taq and Pfu DNA Polymerases.

Components:
D3205: 2’-Deoxythymidine-5’-Triphosphate, Sodium Salt (dTTP) Solution:
Purity (HPLC): 99%
Concentration: 100mM ± 5%
pH: 7.0 ± 0.1
Lambda Max: 260nm
Extinction Coefficient: 9600 (pH 7, 20mM potassium phosphate)
A250/A260: 0.65 ± 0.03 A280/A260: 0.73 ± 0.03
DNase: None Detected RNase: None Detected

D3165: 2’-Deoxyadenosine-5’-Triphosphate, Sodium Salt Solution (dATP):
Purity (HPLC): 99%
Concentration: 100mM ± 5%
pH: 7.0 ± 0.1
Lambda Max: 259nm ± 2nm
Extinction Coefficient: 15200 (pH 7, 20mM potassium phosphate)
A250/A260: 0.80 ± 0.03 A280/A260: 0.15 ± 0.03
DNase: None Detected RNase: None Detected

D3187: 2’-Deoxycytidine-5’-Triphosphate, Sodium Salt (dCTP) Solution:
Purity (HPLC): 99%
Concentration: 100mM ± 5%
pH: 7.0 ± 0.1
Lambda Max: 272nm ± 2nm
Extinction Coefficient: 9300 (pH 7, 20mM potassium phosphate)
A250/A260: 0.80 ± 0.03 A280/A260: 0.96 ± 0.03
DNase: None Detected RNase: None Detected

D3189: 2’-Deoxyguanosine-5’-Triphosphate, Sodium Salt (dGTP) Solution:
Purity (HPLC): 99%
Concentration: 100mM ± 5%
pH: 7.0 ± 0.1
Lambda Max: 253nm
Extinction Coefficient: 13700 (pH 7, 20mM potassium phosphate)
A250/A260: 1.15 ± 0.03 A280/A260: 0.67 ± 0.03
DNase: None Detected RNase: None Detected

Storage and Stability:
May be stored at -20°C for short-term only. For long-term storage, aliquot and store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

™The Polymerase Chain Reaction (PCR) process is covered by patents owned by Hoffman-La Roche.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.