Technical Data
D3200-06
Deoxyribonuclease I (DNase I)
Description:
Deoxyribonuclease I (DNase I) is a Ca 2+ and Mg 2+ dependent endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo-and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Conjugation. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:500 to 1:1500

Optimal dilutions to be detemined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceBovineRabbit
Concentration:
~10mg/ml
Immunogen:
Bovine pancreatic Deoxyribonuclease I
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide, before the addition of 40% glycerol.
Specificity:
Recognizes 31kD bovine Deoxyribonuclease I. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum and well as purified and partially purified deoxyribonuclease I, (bovine pancreas).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.