Technical Data
D3200-07A
Deoxyribonuclease I (DNase I) (Biotin)
Description:
Deoxyribonuclease I (DNase I) is a Ca2+ and Mg2+ dependent endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis.

Applications:
Suitable for use in ELISA, Western Blot, Immunomicroscopy, Immunohistochemistry and Streptavidin or Avidin-based assays. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:2000-1:8000
Optimal dilutions to be detemined by the researcher.

Label:
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)

Biotin/Protein Ratio:
10-20 BAC molecules per Rabbit IgG molecule.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceBovineRabbit
Concentration:
~10mg/ml
Immunogen:
Bovine pancreatic Deoxyribonuclease I
Purity:
Purified by delipidation, salt fractionation and ion exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Conjugated to biotin.
Specificity:
Recognizes bovine Deoxyribonuclease I. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Deoxyribonuclease I (bovine pancreas).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bayer & Wilchek, Methods in Enzymology 184: 138-160 (1990).