Technical Data
D3200-08
Deoxyribonuclease I (DNase I) (HRP)
Description:
Deoxyribonuclease I (DNase I) is a Ca 2+ and Mg 2+ dependent endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo-and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis.

Applications:
Suitable for use in ELISA, Western Blotting, Immunohistochemistry, and Peroxidase-based assays. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:500 to 1:2500
Optimal dilutions to be determined by the researcher.

Label:
Horseradish Peroxidase (HRP).

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1mg4C (-20C Glycerol)Blue IceBovineRabbit
Concentration:
~10mg/ml
Immunogen:
Bovine pancreatic Deoxyribonuclease I
Purity:
Chromatographically purified, delipidated and defibrinated IgG.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% gentamicin sulfate, 10mg/ml BSA, 40% glycerol. Conjugated with horseradish peroxidase.
Specificity:
Recognizes 31kD bovine Deoxyribonuclease I. Crossreactive against mammalian forms. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti peroxidase, anti rabbit serum and purified and partially purified deoxyribonuclease I (bovine pancreas).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.