Technical Data
Deoxyribonuclease II (Deoxyribonuclease-II, DNase II, DNaseII, Deoxyribonuclease 2, Deoxyribonuclease-2, DNase 2, DNase2, Acid DNase, Deoxyribonuclease 2 alpha, DNase2A, DNL, DNL 2, DNL2, Lysosomal DNase II, R31240 2, R31240_2)
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus (1). DNase II causes both chromatin condensation and DNA fragmentation (1). The genes encoding human (2-4), porcine (4), and murine (5) DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (3). DNase II is ubiquitously expressed in human tissues.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000 dilution. Human spleen tissue lysate or THP-1 cell lysate can be used as positive control and an approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
100ug4C Do Not FreezeBlue IceHumanRabbit
As reported
Peptide corresponding to amino acids 347 to 360 of human DNase II precursor.
Purified immunoglobulin
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Recognizes human DNase II.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8 2. Yasuda T, Takeshita H, Iida R, Nakajima T, Hosomi O, Nakashima Y, Kishi K. Molecular cloning of the cDNA encoding human deoxyribonuclease II. J Biol Chem 1998;273:2610-6 3. Krieser RJ, Eastman A. The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis. J Biol Chem 1998;273:30909-14 4. Shiokawa D, Tanuma S. Cloning of cDNAs encoding porcine and human DNase II. Biochem Biophys Res Commun 1998;247:864-9 5. Baker KP, Baron WF, Henzel WJ, Spencer SA. Molecular cloning and characterization of human and murine DNase II. Gene 1998;215:281-9