		Technical Data
D3221-03	Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)

Description:
Desmin (53kD) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle) and their tumors. Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development. In skeletal and cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The exression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in rhabdomyosarcomas and leiomyosarcomas.

Applications:
Suitable for use in Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen, de-paraffinized): 40ug/ml
Immunocytochemistry: 40ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	2Q1104	Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Human Desmin

Purity:

Purified

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

Specificity:

Recognizes human Desmin. Species Crossreactivity: bovine. Does not crossreact with rabbit. Species Sequence Homology: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kehat, I., et al., Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiocytes. The Journal of Clinical Investigation. (2001) 108:407-414.