Technical Data
D3221-05
Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)
Description:
Desmin (53kD) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of Desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal and cardiac muscle cells Desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of Desmin in smooth muscle is much more heterogenous.
Coexpression of Vimentin and Desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard disfunction dramatic changes in the distribution of Desmin have been observed.

Applications:
Suitable for use in Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:50-1:100 using ABC method.
Western Blot: 1:50-1:500
Optimum dilutions to be determined by researcher.

Hybridoma:
Sp2/0-Ag14 myeloma cells with spleen cells from a mouse immunized with a cytoskeletal Desmin extract of chicken gizzard.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b4A44Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceChickenMouse
Concentration:
~0.5mg/ml
Immunogen:
Cytoskeletal Desmin extract of chicken gizzard
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes chicken Desmin, which is expressed in smooth and striated muscle cells and their tumors, i.e. rhabdomyosarcoma and leiomyosarcoma. Species Crossreactivity: Human, mouse, rat, hamster, canine, porcine, rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Pieper, F.R., et al., J. Cell Biol. 108: 1009-1024 (1989). 2. Raats, J.M.H., et al., J. Cell Biol. 111: 1971-1985 (1990). 3. Schaart, G., et al., Development 107: 585-596 (1989). 4. Quax, W., et al., Cell 43: 327-338 (1985). 5. Verhagen, A.P., et al., Prostate 13: 25-38 (1988). 6. Schaart, G., et al., Differentiation 46: 105-115 (1991). 7. Dispersyn, G.D., et al., Cardiovasc. Res. 51: 230-240 (2001). 8. Council, L. and Hameed, O., Modern Pathology 22: 639-650 (2009).