Technical Data
D3221-06
Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)
Description:
Desmin (53kD) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal and cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous.

Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.

Applications:
Suitable for use in Immunoblot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunoblot: 1:50-1:500
Immunohistochemistry (Frozen & paraffin sections): 1:10-1:100; ABC as detection reagent.
Optimum dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG14A45Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Desmin isolated from human leiomyoma
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.065% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added.
Specificity:
Recognizes Desmin. Reactive with human smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.