		Technical Data
D3221-06A	Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)

Description:
Desmin is the main intermediate filament in mature skeletal, cardiac and smooth-muscle cells. Can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas and carcinomas.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen/paraffin): 1:10-1:20 No proteolytic treatment required. (Depending on the secondary system used dilution could be 5-10 times higher, but optimal dilution should always be tested by serial dilution).
Optimal dilution to be determined by researcher.

Positive Control:
Skeletal and smooth muscle myosarcomas

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	5F179	Supernatant

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Isolated from human leiomyoma

Purity:

Supernatant

Form

Supplied as a liquid. Dilute immediately before use with PBS or TBS.

Specificity:

Recognizes the cytoplasmic 53kD human desmin protein. Species Crossreactivity: Most mammals, including mouse, rat and rabbit. Does not crossreact with other intermediate filament proteins and is useful for the identification of myodifferentiation (also in tumors).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Muyen GNP van, et al. Coexpression of Intermediate Filament Polypeptides in Fetal and Adult Tissues. Lab Invest (4) 57:359-369 (1987)