Technical Data
D3221-08
Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)
Description:
Desmin is an intermediate filament protein of both smooth and striated muscles. Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance. Anti-desmin antibody is useful in identification of tumors of myogenic origin.


Applications:
Suitable for use in Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin):1:200 for 30 min at RT. No special pretreatment is required for staining of formalin/paraffin tissues.Optimal dilutions to be determined by the researcher.

Positive Control:
Muscle or Sarcoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanRabbit
Concentration:
~0.2mg/ml
Immunogen:
A synthetic peptide mapping near the C-terminus of human desmin. Cellular localization: Cytoplasmic MW of antigen: 53kD
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide.
Specificity:
Recognizes human desmin. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.