Description:
Desmin is an intermediate filament protein of both smooth and striated muscles. Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance. Anti-desmin antibody is useful in identification of tumors of myogenic origin. It reacts with leiomysarcomas (smooth muscle) as well as rhabdomysarcomas (striated muscle).
Applications:
Suitable for use in Immuofluorescence and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 20 min at RT. No special pretreatment is required prior to staining.
Optimal dilutions to be determined by the researcher.
Positive Control:
Muscle or sarcoma
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1,k | 0.N.247 | Supernatant |
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| Concentration: |
| Not determined |
| Immunogen: |
| Desmin from human muscle. Cellular Localization: Cytoplasmic. MW of Antigen: 53kD |
| Purity: |
| Tissue culture supernatant |
| Form |
| Supplied as a liquid, 0.09% sodium azide. |
| Specificity: |
| Recognizes human Desmin. Species Crossreactivity: Baboon, monkey, bovine, feline, canine, hamster, rat and chicken. |
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| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Van Muijen GN; Ruiter DJ; Warnaar SO. Coexpression of intermediate filament polypeptides in human fetal and adult tissues. Laboratory Investigation, 1987 Oct, 57(4):359-69. 2. Virtanen I; Kallajoki M; Narvanen O; Paranko J; Thornell LE; Miettinen M; Lehto VP. Peritubular myoid cells of human and rat testis are smooth muscle cells that contain desmin-type intermediate filaments. Anatomical Record, 1986 May, 215(1):10-20. 3. Altmannsberger M; Weber K; Droste R; Osborn M. Desmin is a specific marker for rhabdomyosarcomas of human and rat origin. American Journal of Pathology, 1985, 118(1):85-95. 4. Miettinen M; Lehto VP; Virtanen I. Antibodies to intermediate filament proteins. The differential diagnosis of cutaneous tumors. Archives of Dermatology, 1985 Jun, 121(6):736-41. 5. Virtanen I; Miettinen M; Lehto VP; Kariniemi AL; Paasivuo R. Diagnostic application of monoclonal antibodies to intermediate filaments. Annals of the New York Academy of Sciences, 1985, 455:635-48. 6. classification of human tumors. Ultrastructural Pathology, 1984, 7(2-3):83-107. 7. Miettinen M; Lehto VP; Ekblom P; Tasanen A; Virtanen I. Leiomyosarcoma of the mandible: diagnosis as aided by immunohistochemical demonstration of desmin and laminin. Journal of Oral Pathology, 1984 Aug, 13(4):373-81. 8. Miettinen M; Lehto VP; Virtanen I. Antibodies to intermediate filament proteins in the diagnosis and 9. Miettinen M; Lehto VP; Badley RA; Virtanen I. Alveolar rhabdomyosarcoma. Demonstration of the muscle type of intermediate filament protein, desmin, as a diagnostic aid. American Journal of Pathology, 1982 Aug, 108(2):246-51. 10. Miettinen M; Lehto VP; Badley RA; Virtanen I. Expression of intermediate filaments in soft-tissue sarcomas. International Journal of Cancer, 1982 Nov 15, 30(5):541-6.1. Van Muijen GN; Ruiter DJ; Warnaar SO. Coexpression of intermediate filament polypeptides in human fetal and adult tissues. Laboratory Investigation, 1987 Oct, 57(4):359-69. 2. Virtanen I; Kallajoki M; Narvanen O; Paranko J; Thornell LE; Miettinen M; Lehto VP. Peritubular myoid cells of human and rat testis are smooth muscle cells that contain desmin-type intermediate filaments. Anatomical Record, 1986 May, 215(1):10-20. 3. Altmannsberger M; Weber K; Droste R; Osborn M. Desmin is a specific marker for rhabdomyosarcomas of human and rat origin. American Journal of Pathology, 1985, 118(1):85-95. 4. Miettinen M; Lehto VP; Virtanen I. Antibodies to intermediate filament proteins. The differential diagnosis of cutaneous tumors. Archives of Dermatology, 1985 Jun, 121(6):736-41. 5. Virtanen I; Miettinen M; Lehto VP; Kariniemi AL; Paasivuo R. Diagnostic application of monoclonal antibodies to intermediate filaments. Annals of the New York Academy of Sciences, 1985, 455:635-48. 6. classification of human tumors. Ultrastructural Pathology, 1984, 7(2-3):83-107. 7. Miettinen M; Lehto VP; Ekblom P; Tasanen A; Virtanen I. Leiomyosarcoma of the mandible: diagnosis as aided by immunohistochemical demonstration of desmin and laminin. Journal of Oral Pathology, 1984 Aug, 13(4):373-81. 8. Miettinen M; Lehto VP; Virtanen I. Antibodies to intermediate filament proteins in the diagnosis and 9. Miettinen M; Lehto VP; Badley RA; Virtanen I. Alveolar rhabdomyosarcoma. Demonstration of the muscle type of intermediate filament protein, desmin, as a diagnostic aid. American Journal of Pathology, 1982 Aug, 108(2):246-51. 10. Miettinen M; Lehto VP; Badley RA; Virtanen I. Expression of intermediate filaments in soft-tissue sarcomas. International Journal of Cancer, 1982 Nov 15, 30(5):541-6..
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