Technical Data
D3221-10J
Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)
Description:
Desmin is a 53kD intermediate filamentprotein expressed in the cytoplasmic of both smooth and striated muscles. D3221-10J reacts with both striated (skeletal and cardiac) and smooth muscle cells. In skeletal and cardiac muscles, this antibody stains Z-bands giving a characteristic striated appearance. D3221-10J is also useful in identifying tumors of myogenic origin, including rhabdomyosarcomas (striated muscle) and leiomyosarcomas (smooth muscle).

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Immunohistochemistry (frozen): 1:25-1:100
Optimal dilutions to be determined by the researcher.

Positive Control:
Leiomyoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG111E168Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
Not determined.
Immunogen:
Purified desmin from human muscle.
Purity:
Supernatant
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes human Desmin. Species Crossreactivity: baboon, feline, chicken, bovine, canine, hamster, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Altmannsberger et al. Am J Pathol 118: 85, 1985. 2. Miettien et al. Arch Dermatol 121: 736, 1985. 3. Gatter et al. J Clin Pathol 39: 950, 1986.