		Technical Data
D3221-15D	Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)

Description:
Desmin is a muscle protein found in skeletal, cardiac and smooth muscle cells. This antibody will help determine the presence of desmin and identify cells of myogenic origin in uncharacterized tissues.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100-1:250
Immunohistochemistry: 1:50-1:100 for 1 hr. at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Western Blot: Muscle
Immunohistochemistry: Intestine wall (cytoplasmic staining pattern)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	DE-R-11	Supernatant

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice	Porcine	Mouse

Concentration:

Not determined

Immunogen:

Purified porcine desmin.

Purity:

Supernatant

Form

Supplied as a liquid in 15mM sodium azide.

Specificity:

Recognizes porcine Desmin. Species Crossreactivity: Canine, guinea pig, hamster, human and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Altmannsberger M, Weber K, Droste R, et al.. American Journal of Pathology. 118:85-98 (1985). 2. Osborn M, Altmannsberger M, Debus E, et al.. Cancer Cells 1. The transformed phenotype. Cold Spring Harbor Lab. 191-200 (1984). Debus E, Weber K and Osborn M. The EMBO Journal. 2(12):2305-2312 (1983).