Technical Data
D3221-15D
Desmin (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)
Description:
Desmin is a muscle protein found in skeletal, cardiac and smooth muscle cells. This antibody will help determine the presence of desmin and identify cells of myogenic origin in uncharacterized tissues.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:100-1:250
Immunohistochemistry: Frozen sections
Immunohistochemistry (Paraffin): 1:50-1:100 for 1 hour at RT. Trypsin digestion is recommended.
Optimal dilutions to be determined by the researcher.

Positive Control:
Western Blot: Muscle
Immunohistochemistry: Intestine wall (cytoplasmic staining pattern)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1DE-R-11Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceMouse
Concentration:
Not Determined
Immunogen:
Purified porcine desmin.
Purity:
Supernatant
Form
Supplied as a liquid in 15mM sodium azide
Specificity:
Recognizes Desmin. Species Crossreactivity: canine, guinea pig, chicken, mouse, hamster, human and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Altmannsberger M, Weber K, Droste R, et al.. American Journal of Pathology. 118:85-98 (1985). 2. Osborn M, Altmannsberger M, Debus E, et al.. Cancer Cells 1. The transformed phenotype. Cold Spring Harbor Lab. 191-200 (1984). 3. Debus E, Weber K and Osborn M. The EMBO Journal. 2(12):2305-2312 (1983).