Technical Data
D3221-49
Desmocollin 2
Description:
Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal
glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein1-3 and desmocollin1-3. Desmosomal cadherins showed differentiation -specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of desmocollin-2 varies in the basal and suprabasal layers. Desmocollin-3 is expressed more evenly throughout the suprabasal layers. Domain specific desmosomal and adherens junction-associated antibodies have been widely used in recent studies. In one study, domain specific antibodies were used to determine the cellular localization of the skin of patients with Hailey-Hailey disease and Darier’s disease.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 1:100; Incubate for 1hr at RT
Immunohistochemistry (Paraffin-embedded tissue): 1:50, Incubation for >1hr at RT
Western Blot: 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Recombinant corresponding to Extracellular domain of human Desmocollin 2. Cellular Localization: All desmosome-possessing cell types, including epithelia, myocardiac cells, lymph nodes and neoplasia derived lymph nodes.
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes human Desmocollin 2a and 2b (splice forms; MW: 78, 535kD). Species Crossreactivity: bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nuber UA, Schäfer S, Stehr S, Schmidt A, Koch PJ, and Franke WW: The widespread human desmocollin DSC2 and tissue-specific patterns of synthesis of various desmocollin subtypes. Eur. J. Cell Biol. 66: 69-74 (1995). 2. Moll I, Houdek P, Schäfer S, Nuber U, and Moll R: Diversity of desmosomal proteins in regenerating epidermis: Immunohistochemical study using a human skin organ culture model. Arch. Dermatol. Res. 291: 437-446 (1999).