Technical Data
D3221-84
Desmoglein 3
Description:
Desmoglein 3 is a member of the desmosomal cadherin family that comprise the desmogleins and desmocollins. Desmosomes are cell-cell junctions in most epithelial cells and serve to link the intermediate filament cytoskeletons of adjacent cells. Within the desmosome the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 3 is synthesized in both the basal and the lower suprabasal compartments. Domain specific desmosomal and adherens junction-associated antibodies have been widely used in recent studies. In one study, domain specific antibodies were used to determine the cellular localization of the skin of patients with Hailey-Hailey disease and Darier’s disease. Desmosomal cadherin antibodies are also useful for targeting specific disease and in the study of interaction between cell adhesion proteins.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-3ug/ml using ECL
Immunoprecipitation (Native): 5-10ug
Immunohistochemistry (Paraffin): 20-50ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
A431 cells

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13G133Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Human desmoglein 3 extracellular domain.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the human Desmoglein 3 protein at ~140kD. Does not crossreact with desmoglein 1 or desmoglein 2 protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Buxton, R.S., et al., J. Cell Biol. 121: 481-483 (1993). 2. Hakuno, M., et al., Brit. J. Dermatology 142: 702-711 (2000). 3. Kurzen, H., et al., Differentiation 63(5): 295-304 (1998). 4. Proby, C.M., et al., Brit. J. Dermatology 142: 321-330 (2000). 5. Wahl, J.K. III, et al., J. Cell Sci. 113: 1737-1745 (2000).