Technical Data
Media
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Technical Data
Media
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Dextrose Agar (Powder)
D3222
Microbiological Grade
Storage RT/4°C Shipping RT
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Components shown as g/Liter
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Dextrose agar is used for the culture of microorganisms with or without added blood. Supplementation with 5% blood provides additional growth factors for fastidious microorganisms. When pH is lowered to 3.5 medium can be used to obtain total counts of yeasts in food. Directions per Liter: Suspend 43g of the powder in 1L of distilled water. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). Optional: To prepare blood agar, aseptically add 5% sterile defibrinated blood to the medium at 45-50°C. Mix well and dispense as desired. Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time. Appearance: Light to dark tan, homogeneous, freeflowing. Solubility: Amber, clear, complete with heat. pH (43g/L): As Reported Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Norton. 1932. J. Lab. Clin. Med. 17:558. 2. MacFaddin. 1985. Media for isolation-cultivation-identification-maintenance of medical bacteria, vol. 1. Williams & Wilkins, Baltimore, Md. 3. Downes and Ito (ed.). 2001. Compendium of methods for the microbiological examination of foods, 4th ed. American Public Health Association, Washington, D.C. |