Technical Data
Media
Dextrose Agar (Powder)
D3222
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/Liter
Pancreatic Digest of Casein 5.0
Proteose Peptone No. 3 2.0
Pancreatic Digest of Gelatin 3.0
Beef Extract3.0
Dextrose10.0
Sodium Chloride5.0
Agar15.0
Total:43g/L
Dextrose agar is used for the culture of microorganisms with or without added blood. Supplementation with 5% blood provides additional growth factors for fastidious microorganisms. When pH is lowered to 3.5 medium can be used to obtain total counts of yeasts in food.


Directions per Liter: Suspend 43g of the powder in 1L of distilled water. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Optional: To prepare blood agar, aseptically add 5% sterile defibrinated blood to the medium at 45-50C. Mix well and dispense as desired. Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Light to dark tan, homogeneous, freeflowing.

Solubility: Amber, clear, complete with heat.

pH (43g/L): As Reported

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Norton. 1932. J. Lab. Clin. Med. 17:558. 2. MacFaddin. 1985. Media for isolation-cultivation-identification-maintenance of medical bacteria, vol. 1. Williams & Wilkins, Baltimore, Md. 3. Downes and Ito (ed.). 2001. Compendium of methods for the microbiological examination of foods, 4th ed. American Public Health Association, Washington, D.C.