Technical Data
Media
Dextrose Broth (Powder)
D3222-05
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Casein Digest of Peptone10.0
Dextrose5.0
Sodium Chloride5.0
Total:20g/liter
Dextrose fermentation w/o pH indicator.

Description:
Dextrose Broth is a basic medium used to study the fermentation of dextrose by microorganisms. It contains casein peptone used as carbon and nitrogen source and salt for electrolyte balance. Dextrose is the only fermentable carbohydrate. The medium does not contain a pH indicator.

Storage and Stabiity:
Store powdered media at 4C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Directions: 1. Dissolve 20grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi) Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Light beige, homogeneous, free flowing powder.

Solubility: Pale to light yellow,, clear, complete.

pH (25C): 7.30.2

Microbiological Analysis: Escherichia coli ATCC 25922


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.