Dicer functions is a RNase III-like enzyme which cleaves double-stranded RNA into 21-23 nt RNAs (siRNA short interfering RNA). It is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 1:500-1:1000. Detects ~217kD band
Optimal dilutions to be determined by the researcher.
Positive Control: Whole ovary tissue lysate
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|100ug||4°C (-20°C Glycerol)||Blue Ice||Rabbit|
|Synthetic peptide (mouse), corresponding to amino acids FTKHQVLIMTCYVALTVLK (132-150) and QNKQILVPELCAIHPI (1017-1033).|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide, 0.2% gelatin, before the addition of glycerol to 40%.|
|Recognizes mouse Dicer. Species sequence homology: human and rat due to over 90% sequence with immunogen, but testing shows no response in human and rat ovary at the recommended testing conditions.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
General references: 1. Doi N et al. Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors. Curr Biol 13:41-6 (2003). PubMed: 12526743 2. Zhang H et al. Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP. EMBO J 21:5875-85 (2002). PubMed: 12: 411-505|
3. Provost P et al. Dicer is required for chromosome segregation and gene silencing in fission yeast cells. Proc Natl Acad Sci U S A 99:16648-53 (2002). PubMed: 12482946