Dicer functions is a RNase III-like enzyme which cleaves double-stranded RNA into 21-23 nt RNAs (siRNA short interfering RNA). It is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Two transcript variants encoding the same protein have been identified.
Suitable for use in Western Blot. Other applications not tested.
Western Blot: 1:500-1:1000. Detects ~217kD band
Optimal dilutions to be determined by the researcher.
Positive Control: Whole ovary tissue lysate
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|100ug||4°C (-20°C Glycerol)||Blue Ice||Mouse||Rabbit|
|Synthetic peptide corresponding to aa132-150 and 1017-1033 of mouse Dicer.|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.|
|Recognizes mouse Dicer. Species Sequence Homology: human and rat: >90%. Testing demonstrates no response in human and rat ovary at the recommended dilutions.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Doi, N., et al., Short-interfering-RNA-mediated gene silencing in mammalian cells requires Dicer and eIF2C translation initiation factors. Curr. Biol. 13(1): 41-6 (2003, Jan 8). 2. Zhang, H., et al., Human Dicer preferentially cleaves dsRNAs at their termini without a requirement for ATP. EMBO J. 21(21): 5875-85 (2002, Nov 1). 3. Provost, P., et al., Dicer is required for chromosome segregation and gene silencing in fission yeast cells. PNAS USA 99(26): 16648-53 (2002, Dec 24). Epub 2002, Dec 13.|