Technical Data
D3520-01F
Dicer (DICER1, DCR1, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase Motif, HERNA, KIAA0928)
Description:
Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3’ UTR of the target mRNA (3).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG11C1027Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the sequence near the amino terminus of human Dicer.
Purity:
Purified
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogenous levels of total Dicer.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Hutvágner, G. and Zamore, P.D. (2002) Science 297,2056-60. (2) Lee, Y. et al. (2003) Nature 425, 415-9. (3) Diederichs, S. and Haber, D.A. (2007) Cell 131,1097-108.