		Technical Data
D3520-01J	Dicer1, ID (Endoribonuclease Dicer, Helicase with RNase Motif, Helicase-MOI, DICER1, DICER, HERNA, KIAA0928)

Description:
Dicer1 possesses an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide selected from the central region of human Dicer1 (KLH).

Purity:

Purified by ammonium sulfate precipitation.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human Dicer1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Vermeulen, A., et al., RNA 11(5):674-682 (2005). 2.Fukagawa, T., et al., Nat. Cell Biol. 6(8):784-791 (2004). 3.Zhang, H., et al., Cell 118(1):57-68 (2004). 4.Handa, V., et al., Nucleic Acids Res. 31(21):6243-6248 (2003). 5.Kawasaki, H., et al., Nucleic Acids Res. 31(3):981-987 (2003).