		Technical Data
D3680	4,5-Dihydroxytryptamine, Conjugated

Description:
Applications: Suitable for use in ELISA. Other applications not tested.

Specificity
Using a conjugate 4,5-dihydroxytryptamine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:

Compounds Cross-reactivity ratio(a)
4,5-dihydroxytryptamine-G-BSA 1
5,6-dihydroxytryptamine-G-BSA 1/20,000
5-hydroxytryptamine-G-BSA 1/50,000

(a) : 4,5-dihydroxytryptamine-G-BSA concentration/unconjugated or conjugated indolealkylamine concentration at half displacement.
G = Glutaraldehyde, BSA = Bovine Serum Albumin

Recommended dilution
The anti-conjugated 4,5-dihydroxytryptamine antibodies stains well between a 1/2,000-1/5,000 dilution.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice		Rabbit

Concentration:

~1mg/ml

Immunogen:

4,5-dihydroxytryptamine-Glutaraldehyde-BSA

Purity:

Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.

Form

Supplied as a liquid in PBS, pH 7.2. No preservative added.

Specificity:

Recognizes protein-conjugated 4,5-Dihydroxytryptamine. See additional crossreactivity data.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.