Technical Data
D3680
4,5-Dihydroxytryptamine, Conjugated
Description:
Applications: Suitable for use in ELISA. Other applications not tested.

Specificity
Using a conjugate 4,5-dihydroxytryptamine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:

Compounds Cross-reactivity ratio(a)
4,5-dihydroxytryptamine-G-BSA 1
5,6-dihydroxytryptamine-G-BSA 1/20,000
5-hydroxytryptamine-G-BSA 1/50,000

(a) : 4,5-dihydroxytryptamine-G-BSA concentration/unconjugated or conjugated indolealkylamine concentration at half displacement.
G = Glutaraldehyde, BSA = Bovine Serum Albumin

Recommended dilution
The anti-conjugated 4,5-dihydroxytryptamine antibodies stains well between a 1/2,000-1/5,000 dilution.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
4,5-dihydroxytryptamine-Glutaraldehyde-BSA
Purity:
Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity:
Recognizes protein-conjugated 4,5-Dihydroxytryptamine. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.