Technical Data
D3790-01
DJ-1 (PARK7, Parkinson Disease (Autosomal Recessive, Early Onset) 7, FLJ27376, Oncogene DJ1)
Description:
DJ-1 protein has 189 amino acids and was cloned from a HeLa cell cDNA library as a novel oncogene which weakly transformed NIH3T3 cells. Transformation by DJ-1 was enhanced by cotransfection with H-Ras or Myc. The expression of DJ-1 is induced by growth signals and it is translocated from the cytoplasm to the nucleus during the S phase, although no nuclear localization signals are present. It has been suggested that DJ-1 is a mitogen-dependent oncogene product involved in Ras-related signal transduction pathway (1). Northern blotting has shown that DJ-1 is ubiquitously expressed in various human tissues.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunoblot Analysis: 1:1000-1:5000 dilution of this lot detected DJ-1 in RIPA lysates from MCF-7 and LNCaP cells. LNCaP cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-DJ-1 (1:5000 dilution). Proteins were visualized using a goat anti-mouse, IgM secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates DJ-1.
Immunocytochemistry: Has been reported by an independent laboratory to detect DJ-1 in MCF-7, LNCaP and PC-3 cells that had been permeabilized with cold methanol and fixed with formaldehyde.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgM5H62Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
GST fusion protein corresponding to full length human DJ-1.
Purity:
Ascites
Form
Supplied as a liquid with 0.05% sodium azide before the addition of glycerol to 30%.
Specificity:
Recognizes human DJ-1, Mr 20-25kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bonifati, V., et al; Science 299: 256-259, 2003. 2. Cookson, M.R., Neuron 37: 7-10, 2003. 3. Hod, Y., et al; J. Cell. Biochem. 72: 435-444, 1999.
General References:
1. Nagakubo, D., et al., (1997) Biochem. Biophys, Res. Commun. 231: 509-513. 2. Le Naour, F., et al., (2001) Clin Cancer Res 7(11): 3328-3335.