Technical Data
DKK3, 350aa Isoform (REIC, RIG-like 5-6, RIG-like 7-1)
Suitable for use in Western Blot. Other applications not tested.

Recommende Dilution:
Western Blot: 1-3ug/ml; Heterogeneous bands between 45-65kD seen in 293 lysate.
Optimal dilutions to be determined by the researcher.

Positive Control:
293 lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Peptide with sequence AAAALLGGEEI, from C Terminus of the protein sequence according to NP_037385.
Purified from goat serum by ammonium sulfate precipitation followed by immunoaffinity chromatography using the immunizing peptide.
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Recognizes DKK. Expected to recognize the 350aa isoform only. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Krupnik VE et al. Functional and structural diversity of the human Dickkopf gene family. Gene 238 :301-13 (1999). PubMed: 10570958.