Technical Data
Dkk2 (Dickkopf-related Protein 2, Dickkopf-2, Dkk-2, mDkk-2)
Xenopus Dickkopf (Dkk)-1 was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of Dkk have been identified in mammals. Each Dkk molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of Dkk1 and Dkk2 inhibited Wnt-3a-activated signaling, whereas the first Cysrich domains had no effects. In addition, the second Cys-rich domain of Dkk-2, but not that of Dkk-1, was able to activate the canonical pathway in the presence of LRP6, and this LRPdependent signaling does not require Dvl.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceMouseRabbit
Synthetic peptide corresponding to aa241 from mouse Dkk2.
Purified by affinity chromatography.
Supplied as a liquid in PBS, 0.5% BSA, 0.01% thimerosal, 30% glycerol.
Recognizes mouse Dkk2 at ~30-35kD. A ~25kD band can also be detected, presumably to be the cleavage fragment of Dkk2. Species Crossreactivity: human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.