Technical Data
D3795-20
Dkk-4 (DKK4, Dickkopf Gene 4, Dickkopf Homolog 4)
Description:
Xenopus Dickkopf (Dkk) was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of Dkk have been identified in mammals. Each Dkk molecule contains two conserved cysteine-rich domains which are separated by a 50-55 amino acid linker region.

Application(s):
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml; ~30-35kD bands seen in Jurkat lysate.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide aa 210-224, RQHARLRVCQKIEKL, from C Terminal of human DKK4. NP_055235.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5%BSA.
Specificity:
Recognizes human DKK4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Krupnik VE et al. Functional and structural diversity of the human Dickkopf gene family. Gene 238 :301-13 (1999). PubMed: 10570958.