Technical Data
D3810-01N
DKK1 (Dickkopf-related Protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK, UNQ492/PRO1008)
Description:
DKK1 is a 266aa secreted protein with two cysteine rich regions and belonging to the dickkopf family. It is involved in embryonic development through its inhibition of the WNT signaling pathway and in the reentry into the cell cycle of human adult stem cells from bone marrow. Its loss of expression plays a role in development or progression of malignant melanoma. It decreases melanocyte function and is a key player in multiple myeloma bone disease. It is a potential prognostic and diagnostic marker for breast cancer patients with poor prognosis.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
ELISA: 1:128,000
Immunohistochemistry (formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to CDHHQASNSSRLHT, from the C-terminus of the protein sequence according to NP_036374.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes human DKK1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fedi P, Bafico A, Nieto Soria A, Burgess WH, Miki T, Bottaro DP, Kraus MH, Aaronson SA.Isolation and biochemical characterization of the human Dkk-1 homologue, a novel inhibitor of mammalian Wnt signaling.J Biol Chem. 1999 Jul 2;274(27):19465-72. PMID: 10383463.