Technical Data
D3810-01P
DKK1, CT (Dickkopf-related Protein 1, Dickkopf-1, Dkk-1, hDkk-1, SK, UNQ492/PRO1008)
Description:
DKK1 is a 266aa secreted protein with two cysteine rich regions and belonging to the dickkopf family. It is involved in embryonic development through its inhibition of the WNT signaling pathway and in the reentry into the cell cycle of human adult stem cells from bone marrow. Its loss of expression plays a role in development or progression of malignant melanoma. It decreases melanocyte function and is a key player in multiple myeloma bone disease. It is a potential prognostic and diagnostic marker for breast cancer patients with poor prognosis.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:128,000
Western Blot: 1-3ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to human DKK1 at C-terminal (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human DKK1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.