Technical Data
D3810-04
Dkk-2 (DKK2, SK, DKK-2, Dickkopf Homolog 2 (Xenopus laevis), Dickkopf-2 Like, Dickkopf (Xenopus laevis) Homolog 2)
Description:
Dickkopf (DKK) family proteins consist of four members DKK1, DKK2, DKK3 and DKK4 that function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6 (1,2). DKKs contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved (3). Their expression is both temporally and spatially regulated during animal development (4). DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling (5,6). DKK2 plays a role in osteoblast terminal differentiation and formation of mineralized matrix and is required for differentiation of non-keratinizing stratified epithelium from the corneal epithelial progenitors (7,8).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgG
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Immunogen:
Synthetic peptide (KLHcoupled) corresponding to residues surrounding Asn70 of human DKK2.
Purity:
Purified by peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
Specificity:
Detects transfected levels of human DKK2 protein. This antibody does not cross-react with DKK1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Mao, B. et al. (2001) Nature 411, 3215.
(2) Niehrs, C. (2006) Oncogene 25, 746981.
(3) Krupnik, V.E. et al. (1999) Gene 238, 30113.
(4) Monaghan, A.P. et al. (1999) Mech Dev 87, 4556.
(5) Mao, B. et al. (2002) Nature 417, 6647.
(6) Davidson, G. et al. (2002) Development 129, 558796.
(7) Li, X. et al. (2005) Nat Genet 37, 94552.
(8) Mukhopadhyay, M. et al. (2006) Development 133, 214954.