Technical Data
D3878-12
DNA, ds
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Technical Data |
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D3878-12 |
DNA, ds |
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Description: Applications: Suitable for use in EIA and Immunocytochemistry. Other applications not tested. Recommended Dilution: Optimal dilution determined by the researcher. Binding Assay: Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti- DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound (32P) DNA by Chernkov radiation. Antigen Binding Profile: Natural DNA dsDNA(a) ssDNA(b) 1.00 0.45 Synthetic homopolymers poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU). 0.01 0.01 0.01 0.01 0.01 0.01 Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10ug of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0). a) Native calf thymus DNA b) Heat-denatured calf thymus DNA Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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