Technical Data
D3878-12
DNA, ds
Description:
Applications:
Suitable for use in EIA and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilution determined by the researcher.

Binding Assay:
Supernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti- DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound (32P) DNA by Chernkov radiation.

Antigen Binding Profile:
Natural DNA
dsDNA(a) ssDNA(b)
1.00 0.45
Synthetic homopolymers
poly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU).
0.01 0.01 0.01 0.01 0.01 0.01

Nucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10ug of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0). a) Native calf thymus DNA b) Heat-denatured calf thymus DNA

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k2Q2203Supernatant
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Double stranded DNA (dsDNyA) (B-form) from human
Purity:
Tissue culture supernatant
Form
Supplied as a liquid.
Specificity:
Double stranded DNA (dsDNyA) (B-form) from human. Crossreactivity: dsDNA: 95%: ssDNA: 28%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
J. Immunology (1985) 135:33723380. Analytical Biochemistry (1989) 177:259262. Mol. Immunol. (1990) 5:463470. Dev. Biology (1992) 152:94102.