Technical Data
D3878-13B
DNA, ds
Description:
dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Paraformaldehyde fixed or frozen tissue and formalin fixed paraffin-embedded tissue sections.
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil tissue

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG3AE-2Purified
SizeStorageShippingSourceHost
100ug4°C Do Not FreezeBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Nuclei of Raji Burkitt’s lymphoma cells
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes double stranded DNA expressed in the nucleus of human cells. Produces a homogeneous staining pattern in the nucleus of normal and malignant cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In: Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137