Technical Data
D3878-14A
Anti-Double Stranded DNA (dsDNA) BioAssay™ ELISA Kit, Semi-Quantitave
1Kit
Kits and Assays Storage: 4°CShipping: Blue Ice
The United States Biological Anti-dsDNA ELISA Kit is a quantitative assay intended for the detection of antibodies to dsDNA an†igen in human serum as an aid in diagnosis of Systematic Lupus Erythematosus (SLE). This kit is intended for research use only.

Kit Components:
D3878-14A1: Microtiter Plate, 1x96 wells
D3878-14A2: Serum Diluent Type II, 1x30ml
D3878-14A3: Positive Control, 1x400ul D3878-14A4: Calibrator, 1x400ul
D3878-14A5: Negative Control, 1x400ul
D3878-14A6: Horseradish-peroxidase (HRP) Conjugate, 1x16ml
D3878-14A7: Wash Buffer Type I, 20X, 1x50ml.
D3878-14A8: TMB Substrate Solution Type I, 1x15ml
D3878-14A9: Stop Solution, 1x15ml

Note: Serum vials may contain excess volume.

Storage And Stability:
Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
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2. Miniter, M., B. D. Stollar, and V. Agnello. 1979. Reassessment of the clinical significance of native DNA antibodies in systemic lupus erythematosus. Arthritis Rheum. 22:959-968.3. Ruffatti, A., A. Calligaro, T. Del Ross, M.T. Bertoli, A. Doria, L. Rossi, and S. Todesco. 1990. Anti-double-stranded DNA antibodies in the healthy elderly: Prevalence and Characteristics. J. Clin. Immunology. Vol. 10, No. 6. pp. 300-303.4. Wold, R. T., F. E. Young, E. M. Tan, and R. S. Farr. 1968. Deoxyribonucleic acid antibody: a method to detect its primary interaction with deoxyribonucleic acid. Science. 161:806-807.5. Rubin, R. L. 1986. Enzyme-linked immunosorbent assay for anti-DNA and anti-histone antibodies. In: Manual of Clinical Laboratory Immunology. N. R. Rose, H. Friedman, and J. L. Fahey, eds. ASM, Wash., DC. pp. 744-749.6. Henry, J.B. 1991. Immunology and immunopathology. In: Clinical Diagnosis and Management. J. Mitchell, ed. Philadelphia: W.B. Saunder Co. p. 890.7. CDC-NIH Manual. 1993. In: Biosafety in Microbiological and Biomedical Laboratories, 3rd Edition. U. S. Dept. of Health and Human Services, Public Health Service. pp 9-12.8. Engvall, E., and P. Perlman. 1971. Enzyme-Linked Immunosorbent Assay, (ELISA) Quantitative Assay of Immunoglobulin G. Immunochemistry. 8: 871-874.9. Engvall, E., and P. Perlman. 1971. Enzyme-Linked Immunosorbent Assay, ELISA. Peeters. H., ed. In: Protides of the Biological Fluids. Proceedings of the Nineteenth Colloquium, Brugge, Oxford. Pergamon Press. pp 553-556.10. Engvall, E., K. Jonsson, and P. Perlman. 1971. Enzyme- Linked Immunosorbent Assay. II. Quantitative Assay of Protein Antigen, Immunoglobulin-G, By Means of Enzyme- Labelled Antigen and Antibody- Coated tubes. Biochem. Biophys. Acta. 251: 427-434.11. Van Weeman, B. K. and A.H.W.M. Schuurs. 1971. Immunoassay Using Antigen-Enzyme Conjugates. FEBS Letter. 15: 232-235. 12. National Committee for Clinical Laboratory Standards. 1990. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture Approved Standard. NCCLS Publication H18-A. 13. NCCLS. 1991. National Committee for Clinical Laboratory Standard. Internal Quality Control Testing: Principles & Definition. NCCLS Publication C24- A. 14. http://www.cap.org/html/ftpdirectory/checklistftp.html. 1998. Laboratory General - CAP (College of American Pathology) Checklist (April 1998). pp 28-32. 15. NCCLS. 1997. National Committee for Clinical Laboratory Standard. Preparation and Testing of Reagent Water in the Clinical Laboratory. NCCLS Publication C3- A3. 15. NCCLS. 1997. National Committee for Clinical Laboratory Standard. Preparation and Testing of Reagent Water in the Clinical Laboratory. NCCLS Publication C3- A3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.