Technical Data
D3931-61A
DNA Methyltransferase 1 (DNMT, MCMT, M.Hsa1, DNA (cytosine-5-)-methyltransferase 1, DNA MTase Mmu1, M.HsaI)
Description:
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml
Immunohistochemistry (Paraffin): 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Controls: Mouse ES or NIH 3T3 celll lysate (WB), Mouse fibroblasts (IP), Human breast cancer cell line MCF10A (ChIP), Human kidney (IHC)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k4H80Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa637-650 of human Dnmt1 (Genbank Accession No. NP_001370).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human DNA Methyltransferase 1. Species Crossreactivity: mouse and zebrafish
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bestor. T., et al.. J.Mol. Biol. 203: 971-983 (1988). 2. Yen RW, Vertino PM, Nelkin BD et al. Nucl. Acids Res. 20: 2287-2291 (1992). 3. Xie, S. et al. Gene 236: 87-95 (1999). 4. Okano M, Bell DW, Haber DA,a nd Li E. Cell 99: 247-257 (1999). 5. Reik W. et al. J. Nat. Genet 23: 380-382 (1999). 6. Robertson KD, et al. Nat Genet 25(3):338-342 (2000).