		Technical Data
D3931-61R	DNA Methyltransferase 1 (DNMT1, DNMT, AIM, CXXC-type Zinc Finger Protein 9, CXXC9, DNA (Cytosine-5-)-methyltransferase 1, DNA MTase HsaI, FLJ16293, MCMT, M.HsaI, MGC104992)

Description:
DNA methyltransferases (Dnmts) methylates the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.5-1.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Peptide sequence C-RFESPPKTQPTEDN from the internal region of DNMT1 (NP_001370.1).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in Tris-saline, pH 7.3, 0.02% sodium azide, 0.5% BSA.

Specificity:

Recognizes human DNA (cytosine-5)-methyltransferase 1. Species sequence homology: mouse, rat, dog, bovine, pig.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Robertson, K.D. et al. (1999) The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors. Nuc Acids Res. 27: 2291-2298.