Technical Data
D3931-61R
DNA Methyltransferase 1 (DNMT1, DNMT, AIM, CXXC-type Zinc Finger Protein 9, CXXC9, DNA (Cytosine-5-)-methyltransferase 1, DNA MTase HsaI, FLJ16293, MCMT, M.HsaI, MGC104992)
Description:
DNA methyltransferases (Dnmts) methylates the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, genetic imprinting, and X-chromosome inactivation.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:32,000
Western Blot: 0.5-1.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Peptide sequence C-RFESPPKTQPTEDN from the internal region of DNMT1 (NP_001370.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-saline, pH 7.3, 0.02% sodium azide, 0.5% BSA.
Specificity:
Recognizes human DNA (cytosine-5)-methyltransferase 1. Species sequence homology: mouse, rat, dog, bovine, pig.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Robertson, K.D. et al. (1999) The human DNA methyltransferases (DNMTs) 1, 3a and 3b: coordinate mRNA expression in normal tissues and overexpression in tumors. Nuc Acids Res. 27: 2291-2298.