Technical Data
DNA Methyltransferase 1, Human (DNMT1, DNMT, AIM, CXXC-type Zinc Finger Protein 9, CXXC9, DNA (Cytosine-5-)-methyltransferase 1, DNA MTase HsaI, FLJ16293, MCMT, M.HsaI, MGC104992)
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product, E2F1, and HDAC1. Dnmt1 also
cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific
DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6).

Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot:2-4ug/ml
IOptimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k10F697Affinity Purified
100ug-20CBlue IceMouse
Raised against a synthetic peptide corresponding to aa 637-650 of human DNMT1 (Gene accession number NP_001370).
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS containing 0.05% BSA and 0.05% sodium azide.
Recognizes DNMT1. Species Crossreactivity: human, zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bestor T., et al.(1988) J.Mol. Biol. 203: 971-983. 2. Yen R.W., Vertino P.M., Nelkin B.D. et al. (1992) Nucl. Acids Res. 20: 2287-2291. . Xie S. et al. (1999) Gene 236: 87-95. 4. Okano M., Bell D.W., Haber D.A. and Li E. (1999) Cell 99:247-257. 5. Reik W. et al. (1999) J. Nat. Genet 23: 380-382. 6. Robertson K.D., et al. (2000) Nat Genet 25(3):338-342.