Technical Data
D3931-62B
DNA Methyltransferase 2 (Dnmt2, DNA Cytosine 5 Methyltransferase 2 Like Protein 2, DNA Methyltransferase Homolog HsaIIP, PuMet)
Description:
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.

Cellular Localization: Nuclear

Positive Control: mouse testis

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~0.5mg/ml
Immunogen:
This antibody was raised against synthetic peptides corresponding amino acids 39-53 and 361-376 of mouse Dnmt2.
Purity:
Serum
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Specificity:
Species Crossreactivity: Crossreacts with Human and Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yoder JA, Bestor TH. Hum Mol Genet 7(2):279-84 (1998).
2. Okano M, Xie S, Li E.: Nucleic Acids Res 26:2536-40 (1998).