Technical Data
D3931-63
DNA Methyltransferase 2 (Dnmt 2)
Description:
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. Dnmt2 contains all the sequence motifs diagnostic of DNA (cytosine-5)-methyltransferases but appears to lack the large N-terminal regulatory domain common to other eukaryotic methyltransferases.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Mouse Dnmt2 transfected cell lysate was used as a positive control. A protein band with an approximate size of 42kD is detected.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG4H81Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptides corresponding to mouse Dnmt2.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes mouse DNA Methyltransferase 2 (Dnmt 2)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yoder JA, Bestor TH. Hum Mol Genet 7(2):279-84 (1998).
2. Okano M, Xie S, Li E.: Nucleic Acids Res 26:2536-40 (1998).