Technical Data
D3931-63B
DNA Methyltransferase 1 (DNMT, MCMT, M.Hsa1, DNA (cytosine-5-)-methyltransferase 1, DNA MTase Mmu1, M.HsaI)
Description:
Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
Peptide ELISA: 1:32,000.
Western Blot: 0.5-1.5ug/ml. A band at ~170kD band observed in lysates of cell line Jurkat.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See D3931-63B-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
A synthetic peptide from the C-terminus of aa-RFESPPKTQPTEDN.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human DNMT1. Species Crossreactivity: mouse, rat, canine, bovine, porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Dnmt1 deficiency promotes CAG repeat expansion in the mouse germline., Dion V, Lin Y, Hubert L Jr, Waterland RA, Wilson JH., Hum Mol Genet. 2008 May 1;17(9):1306-17. Epub 2008 Feb 5.