Technical Data
Dnmt1 (DNA (cytosine-5)-methyltransferase 1, CXXC-type Zinc Finger Protein 9, DNA Methyltransferase HsaI, DNA MTase HsaI, M.HsaI, MCMT, AIM, CXXC9, DNMT)
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of the Dnmt1 gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.

Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabAffinity Purified
50ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa1603-1616 of human DNMT1 (KLH).
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.1% sodium azide.
Recognizes human DNMT1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.