Technical Data
D3932-11
DNA Plasmid pUC19
50ug
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Technical Data |
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D3932-11 |
DNA Plasmid pUC19 |
50ug |
| Cloning | Storage: -20°CShipping: Blue Ice |
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pUC19 DNA is isolated from E.coli (dam+, dcm+) and purified by ultracentrifugation twice through a cesium chloride gradient in the presence of ethidium bromide. The molecule is a double-stranded circle, 2686 base pairs in length. GenBank/EMBL accession number L09137. pUC19 is a plasmid vector suitable for cloning and for the dideoxy DNA sequencing method. It has multiple cloning sites in the lacZ' region. Since the multiple cloning site is inserted into the 5' end of the lac Z' gene, insertion of foreign DNA in this region will destroy the alpha complementation activity of the lac Z' gene product, making the screening for white colonies on plates containing X-Gal and IPTG possible. Recombinant plasmids generate white colonies while non-recombinant plasmids generate blue colonies. DNA cloned into pUC19 can be end-labeled while still in the plasmid and sequenced without further DNA purification. pUC19 is identical to pUC18 except that the polylinker is inserted in the opposite orientation. pUC19 DNA has a molecular weight of 1.8x10e6 daltons and contains 2686 base pairs. CAP protein binding site – 591-554 (compl. strand); mRNA (LacZ) starts at nt position 507 (compl. strand); lac repressor binding site – 507-487 (compl. strand). Storage and Stability: Store at -20°C. Once thawed, keep at 4°C. During prolonged storage the supercoiled form may be slowly converted into the relaxed circular form. Molecular Weight: 1.74x10e6 Da |
Purity: Purified Concentration: ~0.5mg DNA/ml Form: Supplied as a liquid in 10mM Tris HCl, pH 7.6, 1mM EDTA Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Messing, J., “New M13 Vectors for Cloning”, Methods in Enzymology 101: 20 (1983). 2. Viera, J., and Messing, J., “The pUC Plasmids, M13mp7-derived System for Insertion Mutagenesis and Sequencing with Synthetic Universal Primers”, Gene 19: 259 (1982). 3. Vieira, J., and Messing, J., “Production of Single-Stranded Plasmid DNA”, Methods in Enzymology 153: 3-11 (1987). 4. Yanisch-Perron, C.,Vieira, J., and Messing, J., “Improved M13 Phage Cloning Vectors and Host Strains: Nucleotides Sequences of the M13mp18 and pUC19 Vectors”, Gene 33: 103 (1985).
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