Technical Data
DNA Plasmid pUC19
Cloning Storage: -20°CShipping: Blue Ice
pUC19 DNA is isolated from E. coli (dam+, dcm+). The molecule is a double-stranded closed circular high copy plasmid, 2686 base pairs in length. GenBank/EMBL accession number L09137.

pUC19 is identical to pUC18 except that the polylinker is inserted in the opposite orientation. pUC19 DNA has a molecular weight of 1.74x10e6 daltons.

Storage and Stability: Store at -20°C. Once thawed, keep at 4°C. During prolonged storage the supercoiled form may be slowly converted into the relaxed circular form.

Molecular Weight:
1.74x10e6 Da
Purity: Purified
Concentration: ~0.5mg DNA/ml
Form: Supplied as a liquid in 10mM Tris HCl, pH 7.6, 1mM EDTA

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Messing, J., “New M13 Vectors for Cloning”, Methods in Enzymology 101: 20 (1983). 2. Viera, J., and Messing, J., “The pUC Plasmids, M13mp7-derived System for Insertion Mutagenesis and Sequencing with Synthetic Universal Primers”, Gene 19: 259 (1982). 3. Vieira, J., and Messing, J., “Production of Single-Stranded Plasmid DNA”, Methods in Enzymology 153: 3-11 (1987). 4. Yanisch-Perron, C.,Vieira, J., and Messing, J., “Improved M13 Phage Cloning Vectors and Host Strains: Nucleotides Sequences of the M13mp18 and pUC19 Vectors”, Gene 33: 103 (1985).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.