Technical Data
DNA Polymerase, beta
During base excision repair (BER) DNA polymerase b (Pol b ) is recruited by APE1. Pol b has two distinct domains, the larger is the polymerase domain itself, whereas a small basic N-terminal domain contains an AP lyase activity that excises the abasic sugar-phosphate residue at the strand break. Pol b also interacts with XRCC1, DNA ligase III and poly(ADP-ribose) polymerase in the repair of DNA single strand breaks. Pol b plays a role in both BER pathways, "single-nucleotide" and "long patch". It is the most inaccurate of the six DNA polymerases found in mammalian cells. Overexpression of Pol , as found in some human tumors, could confer an increase in spontaneous mutagenesis. It has been further suggested that Pol b does not excise 5'-oxidized AP sites induced by reactive oxygen species (ROS).

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Not Determined
Recombinant human Pol b.
Supplied as a liquid, 0.05% sodium azide.
Recognizes human DNA Polymerase-beta. Detects a band of ~45kD by Western Blot. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Apurinic endonuclease (Ref-1) is induced in mammalian cells by oxidative stress and involved in clastogenic adaptation: S. Grosch, et al.; Cancer Res. 58, 4410 (1998) 2. Cells deficient in DNA polymerase beta are hypersensitive to alkylating agent-induced apoptosis and chromosomal breakage: K. Ochs, et al.; Cancer Res. 59, 1544 (1999)