Technical Data
D3935-39
DNA Polymerase, iota
Description:
DNA polymerase Iota (pol iota, also known as Rad30B) is a novel human polymerase, unlike any others known. It is an extremely low-fidelity enzyme that is highly error-prone when replicating undamaged and damaged DNA in vitro. To date there is, however, no human disease that can be attributed to overexpression or defects in poli.

Can be used in Western blot at a dilution of 1:5,000.

Positive control: HeLa whole cell extract (100ug) or GM0193 (lymphoblastoid cell line).
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A 15-mer peptide in the C-terminal region of human DNA Polymerase Iota, conjugated to KLH.
Purity:
Serum
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity:
Specific for human DNA Polymerase Iota
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tissier, A. et al. poli, a remarkably error-prone human DNA polymerase. Genes & Dev. 14: 1642-1650 (2000). 2. Tissier, A. et al. Misinsertion and bypass of thymine-thymine dimmers by human DNA polymerase i. EMBO. 19: 5259-5266 (2000). 3. Frank, E.G. et al. Altered nucleotide misinsertion fidelity associated with pol iota-dependent replication at the end of a DNA template. EMBO. 20: 2914-2922 (2001).