Description:
Applications: Suitable for use in Immunofluorescence and ELISA. Other applications have not been tested.
Recommended Dilution: ELISA: 1:100 Optimal dilutions to be determined by the researcher.
Biological Activity: Shows anti-dsDNA activity only in ELISA.
Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Type | Isotype | Clone | Grade |
| Pab | IgG | | Purified |
|
| Size | | Storage | Shipping | Source | Host |
| 2ml | | -20°C | Blue Ice | Human | Human |
|
| Concentration: |
| Not Determined |
| Immunogen: |
| DNA, ss. Derived from donor human serum. |
| Purity: |
| Fractionated from delipidated, defibrinated and precipitated human serum. |
| Form |
| Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O. |
| Specificity: |
| Recognizes human ds-DNA. Exhibits strong positive reaction in an anti-dsDNA ELISA system. Shows no reactivity at a dilution of 1:100 when tested against purified Ro/SS-A, La/SS-B, Sm, nRNP, Scl-70, ssDNA and Jo-1 antigens in an ELISA system designed to detect IgG antibody. Negative for ssDNA in RIA. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
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