Technical Data
D4015
DNA, ds
Description:
Applications:
Suitable for use in Immunofluorescence and ELISA. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:100
Optimal dilutions to be determined by the researcher.

Autoantibody Activity:
Anti-dsDNA Activity by ELISA; 200-400IU/ml (lot-specific). Current lot value: 212IU/ml
Interpretation of Results: <25.0IU/ml: Negative; 25.0-34.9IU/ml: Equivocal; 35.0-99.9IU/ml: Weak Positive; 100-200IU/ml: Moderate Positive; >200IU/ml: Strongly Positive

Anti-dsDNA Activity by ELISA: Interpretation of Results: <16EU/ml: Negative; 16-20EU/ml: Equivocal; Positive; >20EU/ml:
SSA: 4EU/ml
SSB, SCL, Sm: 1EU/ml
Sm/RNP, Jo-1: 2EU/ml

Anti-ssDNA Activity by ELISA: As reported (lot-specific). Current lot value: 225.6U/ml
Interpretation of Results: <68.6U/ml: Negative; 68.6-229U/ml: Moderately Positive; >229U/ml: Strongly Positive

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
2ml-20CBlue IceHumanHuman
Concentration:
200-400IU/ml
Immunogen:
This product is derived from human patient serum.
Purity:
Fractionated from delipidated, defibrinated and precipitated human serum.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O.
Specificity:
Recognizes human ds-DNA. Exhibits strong positive reaction in an anti-dsDNA ELISA system. Shows no reactivity at a dilution of 1:100 when tested against purified Ro/SS-A, La/SS-B, Sm, nRNP, Scl-70, ssDNA and Jo-1 antigens in an ELISA system designed to detect IgG antibody. Negative for ssDNA in RIA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.