Technical Data
D4015-09A
DnaJ (HSP40, HDJ 2)
Description:
HDJ 2 is a novel human homolog of E. colis DNAJ protein from heat shock protein 40 family. HSP40 is involved in chaperonic protein folding. HDJ 2 is cytoplasmic and a nuclear membrane associated protein. Upon heat shock it migrates and becomes associated with the Golgi complex, nucleolus and nuclear membrane. HDJ 2 has a prenylated C-terminus and is not inducible upon heat shock.

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 2ug/mg protein lysate (native and denatured). Use Protein G.
Western Blot: 0.5-1ug/ml for 2hr at RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 min at RT. Staining of formalin/paraffin tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
MAD109, Raji cells. Tonsil.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13F200Affinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
N-terminal puri ed recombinant fragment (aa1-179) of HDJ 2 fusion protein. Cellular Localization: Cytoplasm (ground state) and Golgi complex, nucleolus, and nuclear membrane (upon heat shock). M.W. of Antigen: 44-47kD
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See D4015-09AX.
Specificity:
Recognizes HDJ 2 (DNAJ). Highly specific to human HDJ 2 and shows no crossreactivity with HDJ 1. Associates with a 53kD protein of unknown identity. Species Crossreactivity: monkey, porcine, rabbit, mouse, rat, hamster and bacteria.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sugito, K., et al., Febs Letters 358(2): 161164 (1995). 2. Frydman, J., et al., Nature 370(6485): 111117 (1994). 3. Ohta, T., et al., Journal of Bacteriology 176(15): 47794783 (1994). 4. Chellaiah, A., et al., Biochimica et Biophysica Acta 1174(1): 111-113 (1993). 5. Hattori, H., Journal of Cell Science 104(Pt3): 629638 (1993). 6. Ohtsuka, K., Biochemical and Biophysical Research Communications 197(1): 235240 (1993). 7. Hattori, H., et al., Cell Structure and Function 17(1): 7786 (1992).