Technical Data
DnaJ (HSP40)
DnaJ is a basic 41kD Escherichia coli heat shock protein which belongs to the molecular chaperone class of proteins. The E. coli dnaJ gene is located in an operon with dnaK. The sequence of the dnaJ gene shares identity with eukaryotic cytosol and endoplasmic reticulum DnaJ homologues which are known to be involved in i) protein folding, and membrane translocation of newly synthesized proteins, and ii) initiation of translation. The N-terminal domain of the DnaJ protein constitutes the most highly conserved domain and it is used as the signature sequence of DnaJ family members. This so-called “J” domain is important for DnaJ functions, including interaction with DnaK protein. Bacterial DnaJ protein acts synergistically with the bacterial chaperone DnaK (Hsp70 homologue) and the other co-chaperone GrpE in: 1. Suppressing eukaryotic and prokaryotic polypeptide aggregation, thus promoting protein folding. 2. Facilitating protein translocation through intracellular compartments or protein secretion. 3. Reactivating (“repairing”) some partially aggregated enzymes, such as E. coli RNAP and luciferase. 4. Sequestering s32 E. coli transcription factor from the RNA polymerase core, and probably exposing it in such a way that the s32 is efficiently proteolysed. 5. Activating pre-priming complex during initiation of lambdaDNA replication, and is also involved in the initiation of DNA replication of the P1 and F plasmids.
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot (ECL): 1:1000
Immunoprecipitation: 1:200
Optimal dilutions to be determined by researcher.
Note: For immunoprecipitation, we recommend preclearing the lysate.

Positive Control:
Recombinant E. coli DnaJ Protein

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ul-20°CBlue IceE. coliRabbit
Purified E. coli DnaJ.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Recognizes E. coli DnaJ at ~41kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zylicz, M., Yamamoto, T., McKittrick, N., Sell, S. and Georgopoulos, C. (1985) J. Biol. Chem. 260: 7591-7598.