Technical Data
D4015-09D
DnaJ (HSP40, HDJ 2)
Description:
In prokaryotes, the heat shock response is characterized by the induction of several proteins including DnaJ, DnaK, GroEL and GroES. DnaK is a member of the HSP 70 family, and like other members, exhibits ATPase activity which facilitates the release of bound proteins and disaggregation of protein complexes within the cell. Members of this protein group serve as molecular chaperones. Levels of DnaJ and DnaK are closely linked and, in E. coli, are constituents of a single operon. DnaJ regulates DnaK in several different systems. Relative to DnaK, the level of DnaJ synthesis is quite low.Studies have shown that DnaJ and DnaK, together with GrpE, cooperatively interact with firefly luciferase as it unfolds during thermal denaturation allowing renaturation of the protein upon return to normal temperatures. Also, DnaK together with DnaJ stimulates the binding of RepA initiator protein to the origin of P1 phage in vitro.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgG
SizeStorageShippingSourceHost
100ul-20°CBlue IceE. coliMouse
Concentration:
As reported
Immunogen:
Synthetic peptide: SKTAEEREIRKAYK, corresponding to amino acids 13-27 of E. coli DnaJ.
Purity:
Ascites
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity:
Species Crossreactivity: Crossreacts with E. coli. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Hartl FU et al. Molecular chaperones in protein folding: the art of avoiding sticky situations. Trends Biochem Sci 19:20-5 (1994). Blum P et al. Physiological consequences of DnaK and DnaJ overproduction in Escherichia coli. J Bacteriol 174:7436-44 (1992). Bardwell JC et al. The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein. J Biol Chem 261:1782-5 (1986).