Technical Data
D4016-85
DNER (Delta and Notch-like Epidermal Growth Factor-related Receptor, Brain EGF Repeat Containing Transmembrane Protein, BET)
Description:
DNER, also known as brain EGF repeat containing transmembrane protein (BET), is a neuron-specific type I transmembrane notch ligand that is expressed on membranes of dendrites and cell bodies but not axons. It contains ten extracellular EGF-like domains and one follistatin-like domain (between the 9th and 10th EGF-like domains). DNER mediates neuron-glia interactions and is required for the functional and morphological maturation of the cerebellum. The extracellular domains of human and mouse DNER share 89% amino acid sequence homology.

Applications:
Suitable for use in ELISA, Immunocytochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 5-15ug/ml (used with immersion fixed A172 human glioblastoma cells)
Western Blot: 0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.2mg/ml (upon reconstitution)
Immunogen:
NS0-derived rhDNER (aa 29-637, UniProt Accession # Q8NFT8)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a powder lyophilized from a solution containing PBS with 5% trehalose. Reconstitute to 0.2mg/ml with 500ul sterile PBS.
Specificity:
Recognizes human DNER. In direct ELISAs and Western Blots, this antibody shows ~30% crossreactivity with rmDNER.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.