Technical Data
Deoxyribonuclease II (DNase II) (CT)
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus (1). DNase II causes both chromatin condensation and DNA fragmentation (1). The genes encoding human (2-4), porcine (4), and murine (5) DNase II have been cloned. The DNase II gene encodes a 40kD proenzyme. The mature enzyme consists of two non-identical subunits, the 32 and 12kD (b) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (3). DNase II is ubiquitously expressed in human tissues.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Positive Control:
Tissue lysate of human spleen

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ug-20CBlue IceHumanRabbit
Peptide corresponding to amino acids 347 to 360 of human DNase II precursor (2-4).
Immunoaffinity purified IgG
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes human DNase II (CT), ~32kD band can be detected (alpha chain). Does not recognize the 12kD beta chain.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8. 2. Yasuda T, Takeshita H, Iida R, Nakajima T, Hosomi O, Nakashima Y, Kishi K. Molecular cloning of the cDNA encoding human deoxyribonuclease II. J Biol Chem 1998;273:2610-6. 3. Krieser RJ, Eastman A. The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis. J Biol Chem 1998;273:30909-14 4. Shiokawa D, Tanuma S. Cloning of cDNAs encoding porcine and human DNase II. Biochem Biophys Res Commun 1998;247:864-9. 5. Baker KP, Baron WF, Henzel WJ, Spencer SA. Molecular cloning and characterization of human and murine DNase II. Gene 1998;215:281-9