Technical Data
D6510-40
DHX29 (DEAH (Asp-Glu-Ala-His) Box 29, DDX29, FLJ21492, Nucleic acid helicase DDX)
Description:
DHX29 is an ATP-dependent RNA helicase that belongs to the DEAD-box helicase family (DEAH subfamily). DHX29 contains one central helicase and one helicase at the carboxy-terminal domain (1). Its function has not been fully established but DHX29 was recently shown to facilitate translation initiation on mRNAs with structured 5 untranslated regions (2). DHX29 binds 40S subunits and hydrolyzes ATP, GTP, UTP, and CTP. Hydrolysis of nucleotide triphosphates by DHX29 is strongly stimulated by 43S complexes and is required for DHX29 activity in promoting 48S complex formation (2).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.5-2ug/ml
Western Blot: 0.5-2ug/ml
Immunocytochemistry: 2-50ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18C116Affinity Purified
SizeStorageShippingSourceHost
50ug4C/-20CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Partial sequence of recombinant full-length protein to human DHX29
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Specificity:
Recognizes human DHX29.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
General References:
1. de la Cruz, J. et al. (1999) Trends Biochem Sci 24, 192-8, Pisareva, V.P. et al. (2008) Cell 135, 1237-50.